NPM1 antibody (N-Term)

Catalog No. ABIN2778260

Product Details anti-NPM1 Antibody

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Pig, Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: DMDMSPLRPQ NYLFGCELKA DKDYHFKVDN DENEHQLSLR TVSLGAGAKD

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 77%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NPM1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NPM1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 294 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NPM1 antibody (ABIN2778260)

Komorek, Kuppuswamy, Subramanian, Vijayalingam, Lomonosova, Zhao, Mymryk, Schmitt, Chinnadurai: "Adenovirus type 5 E1A and E6 proteins of low-risk cutaneous beta-human papillomaviruses suppress cell transformation through interaction with FOXK1/K2 transcription factors." in: Journal of virology, Vol. 84, Issue 6, pp. 2719-31, (2010) (PubMed).

Lin, Coutinho-Mansfield, Wang, Pandit, Fu: "The splicing factor SC35 has an active role in transcriptional elongation." in: Nature structural & molecular biology, Vol. 15, Issue 8, pp. 819-26, (2008) (PubMed).

Target Details for NPM1

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM1 (NPM1 Products)

Background: NPM1 is a member of Nucleophosmin (NPM) family. NPM is a ubiquitously expressed nucleolar phosphoprotein that continuously shuttles between the nucleus and cytoplasm
Alias Symbols: B23, NPM
Protein Interaction Partner: COX8A, NPM2, HUWE1, UBC, PA2G4, FUS, TP53, AURKA, SUMO2, SUMO3, ELF4, STAU1, CDKN2A, LGR4, SUMO1, MDM2, RPA3, RPA2, RPA1, ERG, rev, TRMT61A, OSBP, KPNA1, LIMCH1, ARMCX3, YWHAQ, PARK2, TARDBP, TUBA1C, TAB2, CEP152, DZIP1, COPG1, STK38, COPE, PARK7, AKAP11,
Protein Size: 294

Molecular Weight: 33 kDa

Gene ID: 4869

NCBI Accession: NM_002520, NP_002511

UniProt: P06748

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly