NCOA1 antibody (Middle Region)

Catalog No. ABIN2778219

Product Details anti-NCOA1 Antibody

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NCOA1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GLLHNNDRLS DGDSKYSQTS HKLVQLLTTT AEQQLRHADI DTSCKDVLSC

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NCOA1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NCOA1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1399 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Alternative Name: NCOA1 (NCOA1 Products)

Background: NCOA1 acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. NCOA1 binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion.The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alias Symbols: F-SRC-1, KAT13A, MGC129719, MGC129720, NCoA-1, RIP160, SRC1, bHLHe42, bHLHe74
Protein Interaction Partner: ASCC1, NR1I2, PPARG, AR, VDR, THRB, RXRA, RORB, RORA, RARA, HNF4G, NR6A1, NR5A2, ESRRA, ESR1, PAGR1, Esr2, NR3C1, CXADR, PPARA, AHR, KANK2, NCOA2, TRIM24, NCOA1, ANIB1, PGR, COPS5, UBE2L3, TP53, NCOA6, EP300, UBC, RARB, PPARD, RARG, RBBP6, CREBBP, STAT3,
Protein Size: 1399

Molecular Weight: 152 kDa

Gene ID: 8648

NCBI Accession: NM_147223, NP_671756

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha