PARP16 antibody (Middle Region)

Catalog No. ABIN2778107

Product Details anti-PARP16 Antibody

Target: PARP16 (Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Cow, Dog, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARP16 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: PKYFVVTNNQ LLRVKYLLVY SQKPPKSRAS SQLSWFSSHW FTVMISLYLL

Predicted Reactivity: Cow: 79%, Dog: 86%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 86%, Pig: 80%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against PARP16. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PARP16

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 323 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PARP16 antibody (ABIN2778107)

Di Paola, Micaroni, Di Tullio, Buccione, Di Girolamo: "PARP16/ARTD15 is a novel endoplasmic-reticulum-associated mono-ADP-ribosyltransferase that interacts with, and modifies karyopherin-ß1." in: PLoS ONE, Vol. 7, Issue 6, pp. e37352, (2012) (PubMed).

Target Details for PARP16

Target: PARP16 (Poly (ADP-Ribose) Polymerase Family, Member 16 (PARP16))

Alternative Name: PARP16 (PARP16 Products)

Synonyms: PARP16 antibody, zgc:77744 antibody, ARTD15 antibody, C15orf30 antibody, pART15 antibody, BC055447 antibody, C79952 antibody, PARP-16 antibody, LRRGT00109 antibody, RGD1306243 antibody, poly(ADP-ribose) polymerase family member 16 antibody, poly(ADP-ribose) polymerase family member 16 L homeolog antibody, poly (ADP-ribose) polymerase family, member 16 antibody, PARP16 antibody, parp16.L antibody, parp16 antibody, Parp16 antibody

Background: Poly(ADP-ribosyl)ation is an immediate DNA-damage-dependent post-translational modification of histones and other nuclear proteins that contributes to the survival of injured proliferating cells. PARP16 is a member of poly(ADP-ribose) polymerases (PARPs) family that is encoded by different genes and displaying a conserved catalytic domain in which PARP-1 (113 kDa), the founding member, and PARP-2 (62 kDa) are so far the sole enzymes whose catalytic activity has been shown to be immediately stimulated by DNA strand breaks. A large repertoire of sequences encoding novel PARPs now extends considerably the field of poly(ADP-ribosyl)ation reactions to various aspects of the cell biology including cell proliferation and cell death. Some of these new members interact with each other, share common partners and common subcellular localizations suggesting possible fine tuning in the regulation of this post-translational modification of proteins.
Alias Symbols: C15orf30, FLJ20509, FLJ25281, pART15
Protein Interaction Partner: GMCL1, UBC, NEDD4,
Protein Size: 323

Molecular Weight: 36 kDa

Gene ID: 54956

NCBI Accession: NM_017851, NP_060321