MED27 antibody (Middle Region)

Catalog No. ABIN2778099

Product Details anti-MED27 Antibody

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Cow, Rat, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MED27 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LSRPNGTSAM LLVTLGKVLK VIVVMRSLFI DRTIVKGYNE NVYTEDGKLD

Predicted Reactivity: Cow: 100%, Dog: 85%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 92%, Rabbit: 85%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against MED27. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MED27

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 311 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MED27

Target: MED27 (Mediator Complex Subunit 27 (MED27))

Alternative Name: MED27 (MED27 Products)

Background: The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.
Alias Symbols: CRAP34, CRSP34, CRSP8, MGC11274, TRAP37
Protein Interaction Partner: UBC, CDK19, CDK8, MED19, ARFGAP1, MED26, SOX5, POLR2C, RBPJ, AFP, FBXW7, EPAS1, MED4, MED12, MED14, MED1, MED28, OBFC1, MED18, ZC3H13, QKI, TRIP4, TADA2A, MED9, MED30, MED8, MED22, MED10, MED29,
Protein Size: 311

Molecular Weight: 35 kDa

Gene ID: 9442

NCBI Accession: NM_004269, NP_004260

UniProt: Q6P2C8

Pathways: Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha