Claudin 16 antibody (C-Term)

Catalog No. ABIN2778049

Product Details anti-Claudin 16 Antibody

Target: Claudin 16 (CLDN16)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Pig, Rat, Cow, Dog, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Claudin 16 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FLAGAVLTCC LYLFKDVGPE RNYPYSLRKA YSAAGVSMAK SYSAPRTETA

Predicted Reactivity: Cow: 86%, Dog: 86%, Horse: 93%, Human: 100%, Mouse: 86%, Pig: 86%, Rabbit: 93%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against CLDN16. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CLDN16

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 305 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Claudin 16 antibody (ABIN2778049)

Peng, Rao, Adelman, Rizzolo: "Claudin-19 and the barrier properties of the human retinal pigment epithelium." in: Investigative ophthalmology & visual science, Vol. 52, Issue 3, pp. 1392-403, (2011) (PubMed).

Wang, Zhang, Maminishkis, Dong, Zhi, Li, Zhao, Majerciak, Gaur, Chen, Miller: "MicroRNA-204/211 alters epithelial physiology." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 5, pp. 1552-71, (2010) (PubMed).

Target Details for Claudin 16

Target: Claudin 16 (CLDN16)

Alternative Name: CLDN16 (CLDN16 Products)

Synonyms: CLDN16 antibody, HOMG3 antibody, PCLN1 antibody, claudin-16 antibody, Pcln1 antibody, claudin 16 antibody, CLDN16 antibody, Cldn16 antibody

Background: Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. Claudin-16, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in the corresponding gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure.Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in nephrocalcinosis and renal failure. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: HOMG3, PCLN1
Protein Interaction Partner: APP, TJP1,
Protein Size: 305

Molecular Weight: 34 kDa

Gene ID: 10686

NCBI Accession: NM_006580, NP_006571

UniProt: Q9Y5I7

Pathways: Hepatitis C