ING3 antibody (Middle Region)

Catalog No. ABIN2777878

Product Details anti-ING3 Antibody

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ING3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LSSGTGAGAI TMAAAQAVQA TAQMKEGRRT SSLKASYEAF KNNDFQLGKE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 75%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against ING3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ING3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 418 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ING3

Target: ING3 (Inhibitor of Growth Family, Member 3 (ING3))

Alternative Name: ING3 (ING3 Products)

Synonyms: ING3 antibody, Eaf4 antibody, ING2 antibody, MEAF4 antibody, p47ING3 antibody, 1300013A07Rik antibody, P47ING3 antibody, zgc:56327 antibody, inhibitor of growth family member 3 antibody, inhibitor of growth family, member 3 antibody, inhibitor of growth protein 3 antibody, inhibitor of growth family member 3 L homeolog antibody, ING3 antibody, Ing3 antibody, CpipJ_CPIJ009396 antibody, ing3 antibody, ing3.L antibody

Background: ING3 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers.The protein encoded by this gene is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. This protein contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This gene can activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. Overexpression of this gene has been shown to inhibit cell growth and induce apoptosis. Allelic loss and reduced expression of this gene were detected in head and neck cancers. Two alternatively spliced transcript variants encoding different isoforms have been observed.
Alias Symbols: Eaf4, FLJ20089, ING2, p47ING3, MEAF4
Protein Interaction Partner: USHBP1, TRIM54, TEX11, FBXO25, APP, H2AFZ, ELAVL1, NR1H3, NR1H2, DMAP1, MORF4L1, KAT5, RBX1, RUVBL1, CUL1, TRRAP, UBC, SKP2, ACTL6A, EPC1, HHT2, HHT1, MEAF6, EPC2, BRD8, RUVBL2, YEATS4,
Protein Size: 418

Molecular Weight: 47 kDa

Gene ID: 54556

NCBI Accession: NM_019071, NP_061944

UniProt: Q9NXR8