PRDM16 antibody (Middle Region)

Catalog No. ABIN2777828

Product Details anti-PRDM16 Antibody

Target: PRDM16 (PR Domain Containing 16 (PRDM16))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Guinea Pig, Horse, Dog, Cow, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRDM16 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LNHTQDAKLP SPLGNPALPL VSAVSNSSQG TTAAAGPEEK FESRLEDSCV

Predicted Reactivity: Cow: 92%, Dog: 77%, Guinea Pig: 86%, Horse: 85%, Human: 100%, Mouse: 79%, Pig: 92%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against PRDM16. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PRDM16

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1275 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PRDM16

Target: PRDM16 (PR Domain Containing 16 (PRDM16))

Alternative Name: PRDM16 (PRDM16 Products)

Synonyms: PRDM16 antibody, wu:fc09g08 antibody, MEL1 antibody, PFM13 antibody, 5730557K01Rik antibody, C130091E20 antibody, csp1 antibody, mKIAA1675 antibody, mel1 antibody, PR/SET domain 16 antibody, PR domain containing 16 antibody, PRDM16 antibody, prdm16 antibody, Prdm16 antibody

Background: The reciprocal translocation t(1,3)(p36,q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36,q21)-positive MDS/AML. The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal PR domain. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Alias Symbols: KIAA1675, MEL1, PFM13
Protein Interaction Partner: TFAP4, UBC, HDAC2, Ctbp2, SUMO1, SKI, SMAD3, SMAD2, INADL,
Protein Size: 1275

Molecular Weight: 140 kDa

Gene ID: 63976

NCBI Accession: NM_022114, NP_071397

UniProt: Q9HAZ2

Pathways: Stem Cell Maintenance, Brown Fat Cell Differentiation