PURA antibody (Middle Region)

Catalog No. ABIN2777802

Product Details anti-PURA Antibody

Target: PURA (Purine-Rich Element Binding Protein A (PURA))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PURA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: VEFRDYLGDF IEHYAQLGPS QPPDLAQAQD EPRRALKSEF LVRENRKYYM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against PURA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PURA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 322 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PURA

Target: PURA (Purine-Rich Element Binding Protein A (PURA))

Alternative Name: PURA (PURA Products)

Synonyms: CAGER-1 antibody, Pur-alpha antibody, ssCRE-BP antibody, PUR-ALPHA antibody, PUR1 antibody, PURALPHA antibody, zgc:103411 antibody, pur-alpha antibody, pur1 antibody, puralpha antibody, purine-rich element binding protein A antibody, purine rich element binding protein A antibody, purine-rich element binding protein Aa antibody, purine-rich element binding protein A L homeolog antibody, pura antibody, Pura antibody, PURA antibody, puraa antibody, pura.L antibody

Background: PURA is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia.This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: PUR-ALPHA, PUR1, PURALPHA
Protein Interaction Partner: RAF1, UBC, LIN28B, LIN28A, BMI1, TARDBP, IGSF8, ICAM1, IL7R, ZNF638, DDX23, RFC3, RFC2, RB1, PURB, POLA1, CAND1, CUL1, CUL3, EIF2AK2, POU5F1, MEPCE, HNRNPA1, ADAP1, CCNT1, E2F1, SP1, YBX1, CDK5, CNBP, Jvgp5,
Protein Size: 322

Molecular Weight: 35 kDa

Gene ID: 5813

NCBI Accession: NM_005859, NP_005850

UniProt: Q00577