PCCA antibody (N-Term)

Catalog No. ABIN2777736

Product Details anti-PCCA Antibody

Target: PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Horse, Dog, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCCA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LYYSRQCLMV SRNLGSVGYD PNEKTFDKIL VANRGEIACR VIRTCKKMGI

Predicted Reactivity: Cow: 86%, Dog: 86%, Horse: 83%, Human: 100%, Mouse: 77%, Pig: 86%, Rabbit: 86%, Rat: 86%, Zebrafish: 75%

Characteristics: This is a rabbit polyclonal antibody against PCCA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PCCA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 728 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PCCA antibody (ABIN2777736)

Rincón, Aguado, Desviat, Sánchez-Alcudia, Ugarte, Pérez: "Propionic and methylmalonic acidemia: antisense therapeutics for intronic variations causing aberrantly spliced messenger RNA." in: American journal of human genetics, Vol. 81, Issue 6, pp. 1262-70, (2011) (PubMed).

Target Details for PCCA

Target: PCCA (Propionyl CoA Carboxylase, alpha Polypeptide (PCCA))

Alternative Name: PCCA (PCCA Products)

Synonyms: pcca antibody, zgc:66359 antibody, C79630 antibody, wu:fb92g02 antibody, zgc:100925 antibody, propionyl-CoA carboxylase alpha subunit antibody, propionyl-COA carboxylase alpha chain, mitochondrial antibody, propionyl-CoA carboxylase alpha chain antibody, zinc finger CCCH-type containing 13 antibody, propionyl-Coenzyme A carboxylase, alpha polypeptide antibody, propionyl CoA carboxylase, alpha polypeptide antibody, propionyl-CoA carboxylase alpha subunit L homeolog antibody, PCCA antibody, Pcca antibody, BMEI0800 antibody, pccA antibody, NRI_0781 antibody, zc3h13 antibody, pcca antibody, pcca.L antibody

Background: PCCA is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA is the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia.The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: -
Protein Interaction Partner: HUWE1, PARK2, IFIT1, UBE2N, UBC, EBNA-LP, PCCB,
Protein Size: 728

Molecular Weight: 75 kDa

Gene ID: 5095

NCBI Accession: NM_000282, NP_000273

UniProt: Q8WXQ7

Pathways: Monocarboxylic Acid Catabolic Process