SOX18 antibody (Middle Region)

Catalog No. ABIN2777725

Product Details anti-SOX18 Antibody

Target: SOX18 (SRY (Sex Determining Region Y)-Box 18 (SOX18))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOX18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: RTAPPAAPLA GLYYGTLGTP GPYPGPLSPP PEAPPLESAE PLGPAADLWA

Predicted Reactivity: Cow: 86%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SOX18. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SOX18

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 384 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SOX18 antibody (ABIN2777725)

Park, Lee, Park, Choi, Lee, Song, Hwang, Hong, Nakaoka, Makinen, Kim, Alitalo, Hong, Koh: "Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity." in: The Journal of clinical investigation, Vol. 124, Issue 9, pp. 3960-74, (2014) (PubMed).

Deng, Atri, Eichmann, Simons: "Endothelial ERK signaling controls lymphatic fate specification." in: The Journal of clinical investigation, Vol. 123, Issue 3, pp. 1202-15, (2013) (PubMed).

Duong, Proulx, Luciani, Leroux, Detmar, Koopman, Francois: "Genetic ablation of SOX18 function suppresses tumor lymphangiogenesis and metastasis of melanoma in mice." in: Cancer research, Vol. 72, Issue 12, pp. 3105-14, (2012) (PubMed).

Target Details for SOX18

Target: SOX18 (SRY (Sex Determining Region Y)-Box 18 (SOX18))

Alternative Name: SOX18 (SOX18 Products)

Synonyms: HLTS antibody, AI385749 antibody, Ra antibody, Ragl antibody, sox18 antibody, xSox18alpha antibody, xSox18beta antibody, xsox18 antibody, SRY-box 18 antibody, SRY (sex determining region Y)-box 18 antibody, SRY box 18 antibody, SRY-box 18 S homeolog antibody, SRY-box 18 L homeolog antibody, SRY box 1 antibody, SOX18 antibody, Sox18 antibody, sox18.S antibody, sox18.L antibody, Sox1 antibody

Background: SOX18 is a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. This protein plays a role in hair, blood vessel, and lymphatic vessel development. Mutations in this gene have been associated with recessive and dominant forms of hypotrichosis-lymphedema-telangiectasia.
Alias Symbols: HLTS
Protein Interaction Partner: GKAP1, MECP2, MEF2C, HMGB1,
Protein Size: 384

Molecular Weight: 41 kDa

Gene ID: 54345

NCBI Accession: NM_018419, NP_060889

UniProt: P35713