ALF (N-Term) antibody
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- Target
- ALF
- Binding Specificity
- N-Term
- Reactivity
- Human, Rabbit, Rat, Cow, Dog, Guinea Pig, Horse
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- VIPAGRTLPS FTTAELGTSN SSANFTFPGY PIHVPAGVTL QTVSGHLYKV
- Predicted Reactivity
- Cow: 92%, Dog: 92%, Guinea Pig: 83%, Horse: 100%, Human: 100%, Rabbit: 91%, Rat: 100%
- Characteristics
- This is a rabbit polyclonal antibody against ALF. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human ALF
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 453 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- ALF
- Background
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The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.
Alias Symbols: ALF
Protein Interaction Partner: GAB1, NKX2-3, ZBTB3, TLX3, MAFF, RBFOX2, HMG20A, PAX9, SIX6, MAFG, HOXC11, HOXC9, HOXA5, TLX2, EMX2, EMX1, CEBPE, ATF4, ASCL3, GTF2A2, SUB1, ID3, CSNK2A2, CSNK2A1, TBP,
Protein Size: 453 - Molecular Weight
- 50 kDa
- Gene ID
- 11036
- NCBI Accession
- NM_172196, NP_751946
- UniProt
- Q9UNN4
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