PCK1 antibody (Middle Region)

Catalog No. ABIN2777603

Product Details anti-PCK1 Antibody

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Horse, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: NGFFGVAPGT SVKTNPNAIK TIQKNTIFTN VAETSDGGVY WEGIDEPLAS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PCK1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PCK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 622 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1 (PCK1 Products)

Background: PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.
Alias Symbols: PEPCK1, PEPCKC, PEPCK-C
Protein Interaction Partner: UBC, FBXO25, C1R, APP, CUL5, BAG6, TCP1, TCEB1, SKP1, RPS15A, RPS6, RPS3A, RPS3, RPLP0, RPL4, PSMD12, PSMD2, PSMC5, PSMC4, PSMC2, PSMC1, PSMB7, PSMB4, PSMB1, PSMA5, PSMA2, PCK1, LDHA, HSPD1, HSPA1L, DNAJA1, HLA-C, HLA-B, GNAS, GNAI1, GNA12, FASN, EP300, T
Protein Size: 622

Molecular Weight: 69 kDa

Gene ID: 5105

NCBI Accession: NM_002591, NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis