ME1 antibody (N-Term)

Catalog No. ABIN2777597

Product Details anti-ME1 Antibody

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Sheep, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ME1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: QQLNIHGLLP PSFNSQEIQV LRVVKNFEHL NSDFDRYLLL MDLQDRNEKL

Predicted Reactivity: Cow: 92%, Dog: 85%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 91%, Rabbit: 100%, Rat: 100%, Sheep: 92%

Characteristics: This is a rabbit polyclonal antibody against ME1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ME1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 572 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ME1

Target: ME1 (Malic Enzyme 1, NADP(+)-Dependent, Cytosolic (ME1))

Alternative Name: ME1 (ME1 Products)

Background: ME1 is a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: HUMNDME, MES
Protein Interaction Partner: UBC, VTA1, RPUSD2, GORASP2, BOP1, TOM1, NMI, CUL3, UGDH, TBCD, GTF2A1, GARS, CTTN,
Protein Size: 572

Molecular Weight: 64 kDa

Gene ID: 4199

NCBI Accession: NM_002395, NP_002386

UniProt: P48163

Pathways: Regulation of Lipid Metabolism by PPARalpha