FOXP3 antibody (N-Term)

Catalog No. ABIN2777561

Product Details anti-FOXP3 Antibody

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Pig, Horse, Rabbit, Sheep, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: PHFMHQLSTV DAHARTPVLQ VHPLESPAMI SLTPPTTATG VFSLKARPGL

Predicted Reactivity: Cow: 100%, Dog: 79%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 86%, Rat: 86%, Sheep: 100%

Characteristics: This is a rabbit polyclonal antibody against FOXP3. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human FOXP3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 431 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FOXP3 antibody (ABIN2777561)

Kawabata, Ohta, Seiler, Pyle, Ishiguro, Zhang, Becker, Troyer, Tamura: "Naïve rat umbilical cord matrix stem cells significantly attenuate mammary tumor growth through modulation of endogenous immune responses." in: Cytotherapy, Vol. 15, Issue 5, pp. 586-97, (2013) (PubMed).

Takahata, Nomura, Takada, Ohga, Furuno, Hikino, Nakayama, Sakaguchi, Hara: "CD25+CD4+ T cells in human cord blood: an immunoregulatory subset with naive phenotype and specific expression of forkhead box p3 (Foxp3) gene." in: Experimental hematology, Vol. 32, Issue 7, pp. 622-9, (2004) (PubMed).

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: FOXP3 (FOXP3 Products)

Background: Forkhead box protein P3 (FOXP3, Scurfin, Zinc finger protein JM2) encodes a novel member of the forkhead family of transcription factors. It presumably represses transcription, playing a paramount role in determining the amplitude of the response of CD4 T cells to activation
Alias Symbols: JM2, AIID, IPEX, PIDX, XPID, DIETER
Protein Interaction Partner: PRR20A, RBPMS, STUB1, UBC, HSPA1A, IKZF3, GATA3, RUNX1, RELA, NFATC1, HIST1H1B, HDAC1, KAT8, REL, IKZF4, NFAT5, NFATC2, HDAC4, HDAC2, HDAC7, KAT5, HDAC9,
Protein Size: 431

Molecular Weight: 47 kDa

Gene ID: 50943

NCBI Accession: NM_014009, NP_054728

UniProt: Q9BZS1

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation