NOTCH4 antibody (Middle Region)

Catalog No. ABIN2777550

Product Details anti-NOTCH4 Antibody

Target: NOTCH4 (Notch 4 (NOTCH4))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOTCH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: LLLGLGAARE LRDQAGLAPA DVAHQRNHWD LLTLLEGAGP PEARHKATPG

Predicted Reactivity: Cow: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Pig: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against NOTCH4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NOTCH4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 589 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NOTCH4 antibody (ABIN2777550)

Bhat, Sun, Weger, Raouf: "Notch-Induced Expression of FZD7 Requires Noncanonical NOTCH3 Signaling in Human Breast Epithelial Cells." in: Stem cells and development, Vol. 25, Issue 7, pp. 522-9, (2016) (PubMed).

Sun, Klauzinska, Lake, Lee, Santopietro, Raafat, Salomon, Callahan, Artavanis-Tsakonas: "Trp53 regulates Notch 4 signaling through Mdm2." in: Journal of cell science, Vol. 124, Issue Pt 7, pp. 1067-76, (2011) (PubMed).

Sugaya, Sasanuma, Nohata, Kimura, Fukagawa, Nakamura, Ando, Inoko, Ikemura, Mita: "Gene organization of human NOTCH4 and (CTG)n polymorphism in this human counterpart gene of mouse proto-oncogene Int3." in: Gene, Vol. 189, Issue 2, pp. 235-44, (1997) (PubMed).

Target Details for NOTCH4

Target: NOTCH4 (Notch 4 (NOTCH4))

Alternative Name: NOTCH4 (NOTCH4 Products)

Synonyms: INT3 antibody, NOTCH3 antibody, Int-3 antibody, Int3 antibody, N4 antibody, Notch4 antibody, notch 4 antibody, neurogenic locus notch homolog protein 4 antibody, NOTCH4 antibody, Notch4 antibody, LOC100712850 antibody

Background: NOTCH4 is a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. NOTCH4 is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. NOTCH4 functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. NOTCH4 gene may be associated with susceptibility to schizophrenia in a small portion of cases.
Alias Symbols: INT3, NOTCH4
Protein Interaction Partner: EGFL7, Dlg4, SMAD4, SMAD3, SMAD2, TCEB1, UBC, TP53, MDM2, MAML3, MAML2, FBXW7, MAML1, DLL4, PSEN2, NOTCH4, RBPJ, PSEN1,
Protein Size: 589

Molecular Weight: 61 kDa

Gene ID: 4855

Pathways: Notch Signaling