MXI1 antibody (Middle Region)

Catalog No. ABIN2777278

Product Details anti-MXI1 Antibody

Target: MXI1 (MAX Interactor 1 (MXI1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Zebrafish (Danio rerio), Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MXI1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QGPQEMERIR MDSIGSTISS DRSDSEREEI EVDVESTEFS HGEVDNISTT

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 90%, Zebrafish: 77%

Characteristics: This is a rabbit polyclonal antibody against MXI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MXI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 295 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for MXI1

Target: MXI1 (MAX Interactor 1 (MXI1))

Alternative Name: MXI1 (MXI1 Products)

Background: Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The MXI1 gene encodes a transcriptional repressor protein thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in MXI1 are frequently found in patients with prostate tumors. Expression of the c-myc gene, which produces an oncogenic transcription factor, is tightly regulated in normal cells but is frequently deregulated in human cancers. The protein encoded by this gene is a transcriptional repressor thought to negatively regulate MYC function, and is therefore a potential tumor suppressor. This protein inhibits the transcriptional activity of MYC by competing for MAX, another basic helix-loop-helix protein that binds to MYC and is required for its function. Defects in this gene are frequently found in patients with prostate tumors. Three alternatively spliced transcripts encoding different isoforms have been described. Additional alternatively spliced transcripts may exist but the products of these transcripts have not been verified experimentally.
Alias Symbols: MAD2, MGC43220, MXD2, MXI, bHLHc11
Protein Interaction Partner: NOTCH2NL, KRTAP10-3, KRTAP10-8, KRTAP10-5, KRTAP10-9, KRTAP10-7, KRT40, RPL23AP32, CALCOCO2, MAX, ENTPD5, UBC, APP, CDC20, BUB1B, SIN3B, SIN3A, SMC3, MYC,
Protein Size: 295

Molecular Weight: 33 kDa

Gene ID: 4601

NCBI Accession: NM_130439, NP_569157

Pathways: Maintenance of Protein Location