TFAM antibody (N-Term)

Catalog No. ABIN2777277

Product Details anti-TFAM Antibody

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rabbit, Cow, Dog, Guinea Pig, Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TFAM antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AKTTELIRRI AQRWRELPDS KKKIYQDAYR AEWQVYKEEI SRFKEQLTPS

Predicted Reactivity: Cow: 92%, Dog: 83%, Guinea Pig: 75%, Horse: 92%, Human: 100%, Mouse: 75%, Pig: 92%, Rabbit: 83%

Characteristics: This is a rabbit polyclonal antibody against Transcription Factor AntibodiesAM. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TFAM

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 246 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TFAM antibody (ABIN2777277)

Ogasawara, Liu, Pelicano, Hammoudi, Croce, Keating, Huang: "Alterations of mitochondrial biogenesis in chronic lymphocytic leukemia cells with loss of p53." in: Mitochondrion, Vol. 31, pp. 33-39, (2016) (PubMed).

Kemper, Stirone, Krause, Duckles, Procaccio: "Genomic and non-genomic regulation of PGC1 isoforms by estrogen to increase cerebral vascular mitochondrial biogenesis and reactive oxygen species protection." in: European journal of pharmacology, Vol. 723, pp. 322-9, (2014) (PubMed).

Vettor, Valerio, Ragni, Trevellin, Granzotto, Olivieri, Tedesco, Ruocco, Fossati, Fabris, Serra, Carruba, Nisoli: "Exercise training boosts eNOS-dependent mitochondrial biogenesis in mouse heart: role in adaptation of glucose metabolism." in: American journal of physiology. Endocrinology and metabolism, Vol. 306, Issue 5, pp. E519-28, (2014) (PubMed).

Kemper, Zhao, Duckles, Krause: "Endogenous ovarian hormones affect mitochondrial efficiency in cerebral endothelium via distinct regulation of PGC-1 isoforms." in: Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism, Vol. 33, Issue 1, pp. 122-8, (2013) (PubMed).

Teodoro, Duarte, Gomes, Varela, Peixoto, Rolo, Palmeira: "Berberine reverts hepatic mitochondrial dysfunction in high-fat fed rats: a possible role for SirT3 activation." in: Mitochondrion, Vol. 13, Issue 6, pp. 637-46, (2013) (PubMed).

Miyazaki, Iwasawa, Nakashima, Mori, Shigemoto, Nakamura, Katagiri, Takayanagi, Tanaka: "Intracellular and extracellular ATP coordinately regulate the inverse correlation between osteoclast survival and bone resorption." in: The Journal of biological chemistry, Vol. 287, Issue 45, pp. 37808-23, (2012) (PubMed).

Lu, Chen, Zhang, Wang, Luo, Huang: "ZNF143 transcription factor mediates cell survival through upregulation of the GPX1 activity in the mitochondrial respiratory dysfunction." in: Cell death & disease, Vol. 3, pp. e422, (2012) (PubMed).

Walters, Wallace: "Urea cycle gene expression is suppressed by PFOA treatment in rats." in: Toxicology letters, Vol. 197, Issue 1, pp. 46-50, (2010) (PubMed).

Walters, Bjork, Wallace: "Perfluorooctanoic acid stimulated mitochondrial biogenesis and gene transcription in rats." in: Toxicology, Vol. 264, Issue 1-2, pp. 10-5, (2009) (PubMed).

Shi, Burkart, Nicoloro, Czech, Straubhaar, Corvera: "Paradoxical effect of mitochondrial respiratory chain impairment on insulin signaling and glucose transport in adipose cells." in: The Journal of biological chemistry, Vol. 283, Issue 45, pp. 30658-67, (2008) (PubMed).

Target Details for TFAM

Target: TFAM (Transcription Factor A, Mitochondrial (TFAM))

Alternative Name: TFAM (TFAM Products)

Background: The function remains unknown.This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells.
Alias Symbols: TCF6, MtTF1, mtTFA, TCF6L1, TCF6L2, TCF6L3
Protein Interaction Partner: AGTRAP, FBXW11, ARL6IP1, NEDD1, PPP2R1A, GRSF1, UBC, MDM2, SUZ12, EED, PAN2, MYC, FN1, C1QBP, CAND1, COPS5, CUL3, ELAVL1, ICT1, TFB2M, TFB1M, PNP, NRF1,
Protein Size: 246

Molecular Weight: 29 kDa

Gene ID: 7019

NCBI Accession: NM_003201, NP_003192

UniProt: Q00059

Pathways: Chromatin Binding