Cyclin H antibody (N-Term)

Catalog No. ABIN2777257

Product Details anti-Cyclin H Antibody

Target: Cyclin H (CCNH)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Guinea Pig, Rabbit, Horse, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin H antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PHEEMTLCKY YEKRLLEFCS VFKPAMPRSV VGTACMYFKR FYLNNSVMEY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against CCNH. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CCNH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 323 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Cyclin H

Target: Cyclin H (CCNH)

Alternative Name: CCNH (CCNH Products)

Background: Cyclin H regulates CDK7, the catalytic subunit of the CDK- activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive carboxyl-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. It is involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
Alias Symbols: CAK, p34, p37
Protein Interaction Partner: SFN, GOLGA2, SSX2IP, CCDC170, CCDC33, DUSP12, SSSCA1, NDC80, CALCOCO2, PPFIA1, PSMA1, KLC3, RPA3, RPA2, RPA1, NEDD4, CDK7, CDK8, CDK2, CCNC, MNAT1, MBP, CTD, CDK20, CTBP2, CSNK2B, TRIM8, GANAB, RARB, POLR2A, GTF2H4, ERCC2, tat, POLR2B, CDK9, BRCA1, GTF2H1
Protein Size: 323

Molecular Weight: 38 kDa

Gene ID: 902

NCBI Accession: NM_001239, NP_001230

UniProt: P51946

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase