ZEB1 antibody (N-Term)

Catalog No. ABIN2777193

Product Details anti-ZEB1 Antibody

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZEB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: NPRRNNVTNY NTVVETNSDS DDEDKLHIVE EESVTDAADC EGVPEDDLPT

Predicted Reactivity: Cow: 100%, Dog: 100%, Human: 100%, Mouse: 93%, Pig: 100%, Rabbit: 100%, Rat: 93%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against ZEB1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ZEB1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1124 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ZEB1 antibody (ABIN2777193)

Kothandaraman, Bajic, Brendan, Huak, Keow, Razvi, Salto-Tellez, Choolani: "E2F5 status significantly improves malignancy diagnosis of epithelial ovarian cancer." in: BMC cancer, Vol. 10, pp. 64, (2010) (PubMed).

Li, Xia, Huang, Chen, Su, Li, Wang, Ding, Shao: "A strategy to rapidly identify the functional targets of microRNAs by combining bioinformatics and mRNA cytoplasmic/nucleic ratios in culture cells." in: FEBS letters, Vol. 584, Issue 14, pp. 3198-202, (2010) (PubMed).

Target Details for ZEB1

Target: ZEB1 (Zinc Finger E-Box Binding Homeobox 1 (ZEB1))

Alternative Name: ZEB1 (ZEB1 Products)

Background: ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.
Alias Symbols: AREB6, BZP, DELTA-EF1, FECD6, MGC133261, NIL-2-A, NIL-2A, NIL2A, TCF8, ZEB, ZFHEP, ZFHX1A, PPCD3, DELTAEF1
Protein Interaction Partner: SUMO1, CDK6, GTF2A1, UBR4, SUMO2, SIRT1, SOX2, CTBP2, CTBP1, SMAD7, SMAD6, SMARCA4, SERPINH1, SMAD3, EP300, DRAP1, KAT5, SMAD2, SMAD1, CDH1,
Protein Size: 1124

Molecular Weight: 124 kDa

Gene ID: 6935

NCBI Accession: NM_030751, NP_110378

UniProt: P37275

Pathways: Regulation of Muscle Cell Differentiation