Aurora A antibody (C-Term)

Catalog No. ABIN2777171

Product Details anti-Aurora A Antibody

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Binding Specificity: C-Term

Reactivity: Human, Cow, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: ARDLISRLLK HNPSQRPMLR EVLEHPWITA NSSKPSNCQN KESASKQS

Predicted Reactivity: Cow: 80%, Human: 100%, Sheep: 80%

Characteristics: This is a rabbit polyclonal antibody against AURKA. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AURKA

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 403 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Alternative Name: AURKA (AURKA Products)

Background: AURKA is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. It is found at the centrosome in interphase cells and at the spindle poles in mitosis. This protein may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene.
Alias Symbols: AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, PPP1R47
Protein Interaction Partner: CHFR, GADD45A, RPLP2, RPLP0, RPL38, RPL30, RPL27, RPL22, RPL12, RPL11, PSMB7, PSMB5, PSMA6, PSMA5, PSMA1, MAPK6, PPP6C, PCMT1, YBX1, NPM1, NCL, MFAP4, LYZ, ILF3, ILF2, IARS, HSPD1, HSP90AB1, HSP90AA1, HSPA9, HSPA8, HSPA5, HSPA2, HSPA1A, HNRNPU, HNRNPA2B1,
Protein Size: 403

Molecular Weight: 46 kDa

Gene ID: 6790

NCBI Accession: NM_003600, NP_003591

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization