TNNI1 antibody (N-Term)

Catalog No. ABIN2777151

Product Details anti-TNNI1 Antibody

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Cow, Goat, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNNI1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MPEVERKPKI TASRKLLLKS LMLAKAKECW EQEHEEREAE KVRYLAERIP

Predicted Reactivity: Cow: 93%, Dog: 93%, Goat: 79%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TNNI1. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human TNNI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 187 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-TNNI1 antibody (ABIN2777151)

Salzler, Shah, Doré, Bauerlein, Miloscio, Latres, Papadopoulos, Olson, MacDonald, Duan: "Myostatin deficiency but not anti-myostatin blockade induces marked proteomic changes in mouse skeletal muscle." in: Proteomics, Vol. 16, Issue 14, pp. 2019-27, (2016) (PubMed).

Target Details for TNNI1

Target: TNNI1 (Slow Skeletal Troponin I (TNNI1))

Alternative Name: TNNI1 (TNNI1 Products)

Background: Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Each troponin-tropomyosin complex contains 1 Molecule from each of the 3 troponins: T, C, and I. The troponin I (TNI) subfamily contains three genes: TNI-skeletal-fast-twitch, TNI-skeletal-slow-twitch, and TNI-cardiac. TNNI1 prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes.
Alias Symbols: DKFZp451O223, TNN1, SSTNI
Protein Interaction Partner: PNMA1, MDFI, TRIM63, TRIM55, TNNC1, TNNT2, EIF3I, CCDC85B, TNNT1, ACTA1, PKD2L1,
Protein Size: 187

Molecular Weight: 21 kDa

Gene ID: 7135

NCBI Accession: NM_003281, NP_003272

UniProt: P19237