HSD3B1 antibody (N-Term)

Catalog No. ABIN2777011

Product Details anti-HSD3B1 Antibody

Target: HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))

Binding Specificity: N-Term

Reactivity: Human, Rat, Pig, Sheep, Cow, Goat, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD3B1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TGWSCLVTGA GGFLGQRIIR LLVKEKELKE IRVLDKAFGP ELREEFSKLQ

Predicted Reactivity: Cow: 79%, Goat: 82%, Horse: 79%, Human: 100%, Pig: 79%, Rat: 79%, Sheep: 79%

Characteristics: This is a rabbit polyclonal antibody against HSD3B1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSD3B1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 373 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HSD3B1 antibody (ABIN2777011)

Mirkheshti, Park, Jiang, Cropper, Werner, Song, Chatterjee: "Dual targeting of androgen receptor and mTORC1 by salinomycin in prostate cancer." in: Oncotarget, Vol. 7, Issue 38, pp. 62240-62254, (2016) (PubMed).

Target Details for HSD3B1

Target: HSD3B1 (Hydroxy-delta-5-Steroid Dehydrogenase, 3 beta- and Steroid delta-Isomerase 1 (HSD3B1))

Alternative Name: HSD3B1 (HSD3B1 Products)

Background: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Alias Symbols: HSD3B, HSDB3, I, HSDB3A, SDR11E1, 3BETAHSD
Protein Size: 373

Molecular Weight: 42 kDa

Gene ID: 3283

NCBI Accession: NM_000862, NP_000853

UniProt: P14060

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, C21-Steroid Hormone Metabolic Process, Carbohydrate Homeostasis