PKLR antibody (N-Term)

Catalog No. ABIN2776945

Product Details anti-PKLR Antibody

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKLR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: STSIIATIGP ASRSVERLKE MIKAGMNIAR LNFSHGSHEY HAESIANVRE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PKLR. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PKLR

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 543 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PKLR

Target: PKLR (Pyruvate Kinase, Liver and RBC (PKLR))

Alternative Name: PKLR (PKLR Products)

Background: PKLR is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA).The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phohsphoenolpyruvate from pyruvate and ATP. Defects in this enzyme, due to gene mutations or genetic variations, are the common cause of chronic hereditary nonspherocytic hemolytic anemia (CNSHA or HNSHA). Alternatively spliced transcript variants encoding distinct isoforms have been described.
Alias Symbols: PK1, PKL, RPK, PKR, PKRL
Protein Interaction Partner: UBC, WWOX, SUMO4, CLEC4G, PCNA, OTUD5, USPL1, USP3, MYOC, KIF23, ARHGEF6, PXN, PAK1,
Protein Size: 543

Molecular Weight: 58 kDa

Gene ID: 5313

NCBI Accession: NM_181871, NP_870986

Pathways: Ribonucleoside Biosynthetic Process