Leptin antibody (N-Term)

Catalog No. ABIN2776943

Product Details anti-Leptin Antibody

Target: Leptin (LEP)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Goat, Rabbit, Sheep, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MHWGTLCGFL WLWPYLFYVQ AVPIQKVQDD TKTLIKTIVT RINDISHTQS

Predicted Reactivity: Cow: 86%, Dog: 86%, Goat: 91%, Guinea Pig: 79%, Horse: 79%, Human: 100%, Mouse: 100%, Rabbit: 79%, Rat: 100%, Sheep: 91%

Characteristics: This is a rabbit polyclonal antibody against LEP. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LEP

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 167 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Leptin antibody (ABIN2776943)

Müller, Kowalewski, Reichler, Kollár, Balogh: "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." in: Reproduction in domestic animals = Zuchthygiene, Vol. 52 Suppl 2, pp. 187-192, (2017) (PubMed).

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: LEP (LEP Products)

Synonyms: ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody

Background: LEP is a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regula
Alias Symbols: OB, OBS
Protein Interaction Partner: Hk3, SIGLEC6, GHRL, LEPR, UCN, PRKAA2, CLU, CRP, A2M, STAT3,
Protein Size: 167

Molecular Weight: 19 kDa

Gene ID: 3952

NCBI Accession: NM_000230, NP_000221

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process