HSD17B6 antibody (N-Term)

Catalog No. ABIN2776865

Product Details anti-HSD17B6 Antibody

Target: HSD17B6 (Hydroxysteroid (17-Beta) Dehydrogenase 6 (HSD17B6))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSD17B6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MWLYLAAFVG LYYLLHWYRE RQVVSHLQDK YVFITGCDSG FGNLLARQLD

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 79%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against HSD17B6. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HSD17B6

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 317 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-HSD17B6 antibody (ABIN2776865)

Mohler, Titus, Bai, Kennerley, Lih, Tomer, Wilson: "Activation of the androgen receptor by intratumoral bioconversion of androstanediol to dihydrotestosterone in prostate cancer." in: Cancer research, Vol. 71, Issue 4, pp. 1486-96, (2011) (PubMed).

Muthusamy, Andersson, Kim, Butler, Waage, Bergerheim, Gustafsson: "Estrogen receptor ? and 17?-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 50, pp. 20090-4, (2011) (PubMed).

Target Details for HSD17B6

Target: HSD17B6 (Hydroxysteroid (17-Beta) Dehydrogenase 6 (HSD17B6))

Alternative Name: HSD17B6 (HSD17B6 Products)

Synonyms: HSE antibody, RODH antibody, SDR9C6 antibody, 17betaHSD9 antibody, Hsd17b9 antibody, Rdh8 antibody, hydroxysteroid 17-beta dehydrogenase 6 antibody, hydroxysteroid (17-beta) dehydrogenase 6 antibody, HSD17B6 antibody, Hsd17b6 antibody

Background: HSD17B6 has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. HSD17B6 is a member of the retinol dehydrogenase family.The protein encoded by this gene has both oxidoreductase and epimerase activities and is involved in androgen catabolism. The oxidoreductase activity can convert 3 alpha-adiol to dihydrotestosterone, while the epimerase activity can convert androsterone to epi-androsterone. Both reactions use NAD+ as the preferred cofactor. This gene is a member of the retinol dehydrogenase family. Transcript variants utilizing alternative polyadenylation signals exist.
Alias Symbols: HSE, RODH, SDR9C6
Protein Size: 317

Molecular Weight: 35 kDa

Gene ID: 8630

NCBI Accession: NM_003725, NP_003716

UniProt: O14756

Pathways: Steroid Hormone Biosynthesis