Splicing Factor 1 antibody (N-Term)

Catalog No. ABIN2776691

Product Details anti-Splicing Factor 1 Antibody

Target: Splicing Factor 1 (SF1)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: NATPLDFPSK KRKRSRWNQD TMEQKTVIPG MPTVIPPGLT REQERAYIVQ

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SF1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 639 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1 (SF1 Products)

Synonyms: BBP antibody, D11S636 antibody, MBBP antibody, ZCCHC25 antibody, ZFM1 antibody, ZNF162 antibody, BB094781 antibody, CW17R antibody, MZFM antibody, WBP4 antibody, Zfp162 antibody, CG5836 antibody, Dmel\\CG5836 antibody, cg5836 antibody, dSF1 antibody, p70 antibody, SF1 antibody, sf1 antibody, wu:fc09f06 antibody, znf162 antibody, MWD22.25 antibody, MWD22_25 antibody, SF-1 antibody, splicing factor 1 antibody, Splicing factor 1 antibody, splicing factor-like protein antibody, nuclear receptor subfamily 5 group A member 1 antibody, splicing factor 1 S homeolog antibody, SF1 antibody, Sf1 antibody, sf1 antibody, AT5G51300 antibody, NR5A1 antibody, sf1.S antibody

Background: SF1 contains 1 KH domain and 1 CCHC-type zinc finger. It is necessary for the ATP-dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. It may act as transcription repressor.
Alias Symbols: BBP, MBBP, ZFM1, ZNF162, D11S636
Protein Interaction Partner: KRTAP26-1, TRIM69, FAM168A, CIAO1, MKRN3, PSMA3, FUS, UBC, NEDD8, SUZ12, EED, RPL24, PLOD2, OSBP, NARS, GFPT1, GART, EIF4B, APBB1, SUPT5H, TROVE2, ITCH, PRPF40A, SAMHD1, XPO7, WBP4, FAF1, TCERG1, HSPH1, FARSB, GAS7, RANBP3, HIRIP3, HIST1H4A, XRCC5, U2AF2,
Protein Size: 639

Molecular Weight: 68 kDa

Gene ID: 7536

NCBI Accession: NM_004630, NP_004621

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location