Splicing Factor 1 antibody (C-Term)

Catalog No. ABIN2776689

Product Details anti-Splicing Factor 1 Antibody

Target: Splicing Factor 1 (SF1)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Splicing Factor 1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MASSTPLPWQ QNTTTTTTSA GTGSIPPWQQ QQAAAAASPG APQMQGNPTM

Predicted Reactivity: Cow: 100%, Dog: 100%, Goat: 92%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 639 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for Splicing Factor 1

Target: Splicing Factor 1 (SF1)

Alternative Name: SF1 (SF1 Products)

Background: SF1 contains 1 KH domain and 1 CCHC-type zinc finger. It is necessary for the ATP-dependent first step of spliceosome assembly and binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. It may act as transcription repressor.
Alias Symbols: D11S636, ZFM1, ZNF162, BBP, MBBP
Protein Interaction Partner: KRTAP26-1, TRIM69, FAM168A, CIAO1, MKRN3, PSMA3, FUS, UBC, NEDD8, SUZ12, EED, RPL24, PLOD2, OSBP, NARS, GFPT1, GART, EIF4B, APBB1, SUPT5H, TROVE2, ITCH, PRPF40A, SAMHD1, XPO7, WBP4, FAF1, TCERG1, HSPH1, FARSB, GAS7, RANBP3, HIRIP3, HIST1H4A, XRCC5, U2AF2,
Protein Size: 639

Molecular Weight: 68 kDa

Gene ID: 7536

NCBI Accession: NM_004630, NP_004621

UniProt: Q15637

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Maintenance of Protein Location