NXF5 antibody (Middle Region)

Catalog No. ABIN2776599

Product Details anti-NXF5 Antibody

Target: NXF5 (Nuclear RNA Export Factor 5 (NXF5))

Binding Specificity: Middle Region

Reactivity: Human, Dog, Rabbit, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NXF5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: ITERNFPELL SLNLCNNKLY QLDGLSDITE KAPKVKTLNL SKNKLESAWE

Predicted Reactivity: Dog: 86%, Human: 100%, Mouse: 79%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against NXF5. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NXF5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 397 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for NXF5

Target: NXF5 (Nuclear RNA Export Factor 5 (NXF5))

Alternative Name: NXF5 (NXF5 Products)

Synonyms: NXF2B antibody, nuclear RNA export factor 5 antibody, nuclear RNA export factor 2 antibody, NXF5 antibody, LOC609849 antibody, Nxf5 antibody

Background: NXF5 is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity.This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. Five transcript variants that encode different isoforms have been found for this gene.
Protein Interaction Partner: NXF1,
Protein Size: 397

Molecular Weight: 44 kDa

Gene ID: 55998

NCBI Accession: NM_032946, NP_116564

UniProt: Q9H1B4