EIF2A antibody (C-Term)

Catalog No. ABIN2776588

Product Details anti-EIF2A Antibody

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Guinea Pig, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: DKSPDLAPTP APQSTPRNTV SQSISGDPEI DKKIKNLKKK LKAIEQLKEQ

Predicted Reactivity: Cow: 86%, Dog: 100%, Goat: 83%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against EIF2A. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human EIF2A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 585 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF2A antibody (ABIN2776588)

Feng, Chen, Chan, Chen: "Therapeutic Role of Protein Tyrosine Phosphatase 1B in Parkinson's Disease via Antineuroinflammation and Neuroprotection In Vitro and In Vivo." in: Parkinson's disease, Vol. 2020, pp. 8814236, (2020) (PubMed).

Xu, Liu, Caffaro, Hirschberg: "Inhibition of Golgi apparatus glycosylation causes endoplasmic reticulum stress and decreased protein synthesis." in: The Journal of biological chemistry, Vol. 285, Issue 32, pp. 24600-8, (2010) (PubMed).

Su, Wang, Baltzis, Qu, Wong, Koromilas: "Tyrosine phosphorylation acts as a molecular switch to full-scale activation of the eIF2alpha RNA-dependent protein kinase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 1, pp. 63-8, (2006) (PubMed).

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: EIF2A (EIF2A Products)

Synonyms: ATEIF2-A2 antibody, EIF2-A2 antibody, eukaryotic translation initiation factor 2 alpha subunit antibody, EIF-2A antibody, MST089 antibody, MSTP004 antibody, MSTP089 antibody, D030048D22 antibody, D3Ertd194e antibody, im:7145971 antibody, zgc:110460 antibody, DDBDRAFT_0185936 antibody, DDBDRAFT_0234177 antibody, DDB_0185936 antibody, DDB_0234177 antibody, Eif2a antibody, eif2a antibody, eif2alpha antibody, EIF2A antibody, CG9946 antibody, Dmel\\CG9946 antibody, Eif2alpha antibody, IF2A_DROME antibody, M(1)14C antibody, M(1)19-153 antibody, M(1)8e3-10 antibody, deIF2alpha antibody, eIF-2 antibody, eIF-2 a antibody, eIF-2 alpha antibody, eIF2 alpha antibody, eIF2-alpha antibody, eIF2a antibody, eIF2alpha antibody, eiF-2alpha antibody, elF2alpha antibody, l(1)14Cf antibody, l(1)19-153 antibody, eukaryotic translation initiation factor 2 alpha subunit antibody, eukaryotic translation initiation factor 2A antibody, eukaryotic translation initiation factor 2A, 65kDa antibody, eukaryotic translation initiation factor 2A S homeolog antibody, eukaryotic translation initiation factor antibody, eukaryotic translation Initiation Factor 2alpha antibody, EIF2 ALPHA antibody, EIF2A antibody, Eif2a antibody, eif2a antibody, eIF2a antibody, eif2a.S antibody, eif2A antibody, eIF-2alpha antibody

Background: EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes.EIF2A is a 65-kD protein that catalyzes the formation of puromycin-sensitive 80S preinitiation complexes (Zoll et al., 2002).[supplied by OMIM].
Alias Symbols: CDA02, MST089, MSTP004, MSTP089, EIF-2A
Protein Interaction Partner: UBC, CDC20, RPS23, RPS19, RPS16, RPS10, RPS7, RPS5, HDLBP, PRUNE2, EIF3K, SERBP1, RPS29, RPS24, EIF2AK2, MLH1, NXF1, ILF3, PA2G4, APP, PPP1CC, NEDD8, DCC, ATF4,
Protein Size: 585

Molecular Weight: 64 kDa

Gene ID: 83939

NCBI Accession: NM_032025, NP_114414

UniProt: Q9BY44

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly