Zona Pellucida Glycoprotein 3 antibody (C-Term)

Catalog No. ABIN2776494

Product Details anti-Zona Pellucida Glycoprotein 3 Antibody

Target: Zona Pellucida Glycoprotein 3 (ZP3)

Binding Specificity: C-Term

Reactivity: Human, Rat, Cow, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Zona Pellucida Glycoprotein 3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: NKGDCGTPSH SRRQPHVMSQ WSRSASRNRR HVTEEADVTV GATDLPGQEW

Predicted Reactivity: Cow: 100%, Horse: 100%, Human: 100%, Rabbit: 100%, Rat: 90%

Characteristics: This is a rabbit polyclonal antibody against ZP3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ZP3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 424 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Zona Pellucida Glycoprotein 3 antibody (ABIN2776494)

Amorim, Moya, Donnez, Dolmans: "Morphometric characteristics of preantral and antral follicles and expression of factors involved in folliculogenesis in ovaries of adult baboons (Papio anubis)." in: Journal of assisted reproduction and genetics, Vol. 33, Issue 5, pp. 617-626, (2016) (PubMed).

Törmälä, Jääskeläinen, Lakkakorpi, Liakka, Tapanainen, Vaskivuo: "Zona pellucida components are present in human fetal ovary before follicle formation." in: Molecular and cellular endocrinology, Vol. 289, Issue 1-2, pp. 10-5, (2008) (PubMed).

Target Details for Zona Pellucida Glycoprotein 3

Target: Zona Pellucida Glycoprotein 3 (ZP3)

Alternative Name: ZP3 (ZP3 Products)

Synonyms: ZP3A antibody, ZP3B antibody, ZPC antibody, Zp-3 antibody, zp3.2 antibody, Zp-3B antibody, zona pellucida glycoprotein 3 antibody, zona pellucida sperm-binding protein 3 antibody, ZP3 antibody, zp3 antibody, Zp3 antibody

Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The protein encoded by this gene is a structural component of the zona pellucida and functions in primary binding and induction of the sperm acrosome reaction. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a C-terminal consensus furin cleavage site, and a transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. A variation in the last exon of this gene has previously served as the basis for an additional ZP3 locus, however, sequence and literature review reveals that there is only one full-length ZP3 locus in the human genome. Another locus encoding a bipartite transcript designated POMZP3 contains a duplication of the last four exons of ZP3, including the above described variation, and maps closely to this gene
Alias Symbols: ZPC, ZP3A, ZP3B, Zp-3
Protein Interaction Partner: HECW2, OR2L13, SPACA3, MICALL1, MRPS16, PCDH17, UBAP2L, UBC, ENGASE, ACR, FURIN,
Protein Size: 424

Molecular Weight: 47 kDa

Gene ID: 7784

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process