POLR2I antibody (N-Term)
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- Target See all POLR2I Antibodies
- POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Dog, Rabbit, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This POLR2I antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- ACRNCDYQQE ADNSCIYVNK ITHEVDELTQ IIADVSQDPT LPRTEDHPCQ
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%, Zebrafish: 93%
- Characteristics
- This is a rabbit polyclonal antibody against POLR2I. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the N terminal region of human POLR2I
- Top Product
- Discover our top product POLR2I Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 125 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- POLR2I (Polymerase (RNA) II (DNA Directed) Polypeptide I, 14.5kDa (POLR2I))
- Alternative Name
- POLR2I (POLR2I Products)
- Synonyms
- RPB9 antibody, hRPB14.5 antibody, GB18029 antibody, RPB14.5 antibody, 2810002B19Rik antibody, zgc:103515 antibody, RNA polymerase II subunit I antibody, DNA-directed RNA polymerase II subunit RPB9 antibody, DNA-directed RNA polymerase II 14.5 kDa polypeptide antibody, polymerase (RNA) II (DNA directed) polypeptide I antibody, polymerase (RNA) II subunit I L homeolog antibody, POLR2I antibody, LOC412547 antibody, LOC100282092 antibody, Polr2i antibody, polr2i antibody, polr2i.L antibody
- Background
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POLR2I is a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. POLR2I has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. The product of this gene has two zinc finger motifs with conserved cysteines and the subunit does possess zinc binding activity. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: RPB9, hRPB14.5
Protein Interaction Partner: ESR1, TCEB3, SRC, PSMB9, UBC, MED19, MED26, RECQL5, TBCD, BRCA1, CPSF6, POLR2E, POLR2D, POLR2C, POLR2B, POLR2A, DCUN1D1, CUL5, MYC, tat, WDR48, POLR2H, MED10, MED29,
Protein Size: 125 - Molecular Weight
- 14 kDa
- Gene ID
- 5438
- NCBI Accession
- NM_006233, NP_006224
- UniProt
- P36954
- Pathways
- Regulatory RNA Pathways
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