EED antibody (N-Term)

Catalog No. ABIN2776407

Product Details anti-EED Antibody

Target: EED (Embryonic Ectoderm Development (EED))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EED antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Sequence: GDENDDAVSI ESGTNTERPD TPTNTPNAPG RKSWGKGKWK SKKCKYSFKC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against EED. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EED

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 441 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EED antibody (ABIN2776407)

Lin, Huang, Chang, Chang, Yen, Lee, Chang, Liu, Chang: "Long noncoding RNA TUG1 is downregulated in non-small cell lung cancer and can regulate CELF1 on binding to PRC2." in: BMC cancer, Vol. 16, pp. 583, (2016) (PubMed).

Rakotobe, Violot, Hong, Gouet, Boulanger: "Mapping of immunogenic and protein-interacting regions at the surface of the seven-bladed beta-propeller domain of the HIV-1 cellular interactor EED." in: Virology journal, Vol. 5, pp. 32, (2008) (PubMed).

Target Details for EED

Target: EED (Embryonic Ectoderm Development (EED))

Alternative Name: EED (EED Products)

Synonyms: Xeed antibody, heed antibody, wait1 antibody, EED antibody, HEED antibody, WAIT1 antibody, zgc:112509 antibody, Xeed-B antibody, eed antibody, psXEED antibody, ENSMUSG00000039373 antibody, l(7)5Rn antibody, l7Rn5 antibody, lusk antibody, Xeed-A antibody, embryonic ectoderm development antibody, embryonic ectoderm development S homeolog antibody, microRNA 6755 antibody, embryonic ectoderm development L homeolog antibody, eed antibody, EED antibody, eed.S antibody, MIR6755 antibody, Eed antibody, eed.L antibody

Background: EED is a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein interacts with enhance
Alias Symbols: HEED, WAIT1
Protein Interaction Partner: PINK1, BRCA1, ADAM17, EZH2, IMP3, PSPC1, NAT10, PBRM1, RIF1, RBM28, HEATR1, MAGOHB, ARGLU1, MRPL20, NRDE2, CASZ1, BCOR, EXOSC4, LUC7L3, SF3B6, NOP58, SRRT, RAB14, C9orf114, CRNKL1, DDX47, HP1BP3, MYEF2, TRA2A, CPSF1, IGHV3-23, UTP20, PRPF19, ZNF638, RBMX,
Protein Size: 441

Molecular Weight: 50 kDa

Gene ID: 8726

NCBI Accession: NM_003797, NP_003788

UniProt: O75530