PITX2 antibody (N-Term)

Catalog No. ABIN2776373

Product Details anti-PITX2 Antibody

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Pig, Rabbit, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PITX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: SAVSSSSCHH PQPLAMASVL APGQPRSLDS SKHRLEVHTI SDTSSPEAAE

Predicted Reactivity: Cow: 100%, Dog: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against PITX2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PITX2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 324 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PITX2 antibody (ABIN2776373)

Hatou, Yoshida, Higa, Miyashita, Inagaki, Okano, Tsubota, Shimmura: "Functional corneal endothelium derived from corneal stroma stem cells of neural crest origin by retinoic acid and Wnt/?-catenin signaling." in: Stem cells and development, Vol. 22, Issue 5, pp. 828-39, (2013) (PubMed).

Target Details for PITX2

Target: PITX2 (Paired-Like Homeodomain 2 (PITX2))

Alternative Name: PITX2 (PITX2 Products)

Background: The PITX2 gene encodes a member of the RIEG/PITX homeobox family, which is in the bicoid class of homeodomain proteins. This protein acts as a transcription factor and regulates procollagen lysyl hydroxylase gene expression. Mutations in PITX2 are associated with Axenfeld-Rieger syndrome (ARS), iridogoniodysgenesis syndrome (IGDS), and sporadic cases of Peters anomaly. This protein is involved in the development of the eye, tooth and abdominal organs. It also acts as a transcriptional regulator involved in basal and hormone-regulated activity of prolactin.
Alias Symbols: RS, RGS, ARP1, Brx1, IDG2, IGDS, IHG2, PTX2, RIEG, IGDS2, IRID2, Otlx2, RIEG1
Protein Interaction Partner: LEF1, Hoxa1, WDR5, SMAD3, HDAC1, CTNNB1, ZNHIT3, TRIM25, HERC5, PDLIM1, PITX2, PROP1, KAT5, Pou1f1, MSX2,
Protein Size: 324

Molecular Weight: 36 kDa

Gene ID: 5308

NCBI Accession: NM_000325, NP_000316

Pathways: Retinoic Acid Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development