ZYX antibody (Middle Region)

Catalog No. ABIN2775767

Product Details anti-ZYX Antibody

Target: ZYX (Zyxin (ZYX))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Cow, Horse, Rabbit, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZYX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: QPRGPPASSP APAPKFSPVT PKFTPVASKF SPGAPGGSGS QPNQKLGHPE

Predicted Reactivity: Cow: 86%, Dog: 93%, Guinea Pig: 86%, Horse: 93%, Human: 100%, Rabbit: 79%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against ZYX. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ZYX

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 572 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ZYX

Target: ZYX (Zyxin (ZYX))

Alternative Name: ZYX (ZYX Products)

Synonyms: BcDNA:LD06023 antibody, CG32018 antibody, DLim-1 antibody, DmLIM-1 antibody, Dmel\\CG32018 antibody, Lim102EF antibody, ZYX102 antibody, Zyx102 antibody, Zyx102EF antibody, lim antibody, zyx102 antibody, zyxin antibody, zgc:171680 antibody, ZYX antibody, zyx antibody, MGC145270 antibody, ESP-2 antibody, HED-2 antibody, 9530098H06Rik antibody, R75157 antibody, Zyxin antibody, zyxin antibody, zyxin S homeolog antibody, zyx-1 antibody, Zyx antibody, zyx antibody, ZYX antibody, EDI_201420 antibody, zyx.S antibody

Background: TRIM50 is an E3 ubiquitin-protein ligase.Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
Alias Symbols: ESP-2, HED-2
Protein Interaction Partner: RTN4IP1, YPEL3, IMP3, PHF21A, PDLIM5, LIN7A, SUMO1, NEDD8, FAM129B, CORO1B, CAND1, PUF60, PAPOLA, NUDC, SEC23B, SEC24C, HIST1H4A, UGP2, TARS, PXN, GART, GARS, APEH, FN1, HIPK3, VCAM1, UBC, UBL7, UFC1, UBXN7, ZC3H7B, TTLL12, FERMT2, TOMM34, AHCYL1, SSSCA1,
Protein Size: 572

Molecular Weight: 61 kDa

Gene ID: 7791

NCBI Accession: NM_003461, NP_003452

UniProt: Q15942