CCT4 antibody (C-Term)

Catalog No. ABIN2775694

Product Details anti-CCT4 Antibody

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Saccharomyces cerevisiae, Goat, Zebrafish (Danio rerio), Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: AFADAMEVIP STLAENAGLN PISTVTELRN RHAQGEKTAG INVRKGGISN

Predicted Reactivity: Cow: 93%, Dog: 93%, Goat: 79%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%, Yeast: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CCT4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human CCT4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 539 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CCT4 antibody (ABIN2775694)

Tarkar, Loges, Slagle, Francis, Dougherty, Tamayo, Shook, Cantino, Schwartz, Jahnke, Olbrich, Werner, Raidt, Pennekamp, Abouhamed, Hjeij, Köhler, Griese, Li, Lemke, Klena, Liu, Gabriel, Tobita et al.: "DYX1C1 is required for axonemal dynein assembly and ciliary motility. ..." in: Nature genetics, Vol. 45, Issue 9, pp. 995-1003, (2013) (PubMed).

Machida, Masutani, Kobayashi, Mikami, Nishino, Miyazawa, Imataka: "Reconstitution of the human chaperonin CCT by co-expression of the eight distinct subunits in mammalian cells." in: Protein expression and purification, Vol. 82, Issue 1, pp. 61-9, (2012) (PubMed).

Target Details for CCT4

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Alternative Name: CCT4 (CCT4 Products)

Synonyms: zgc:65789 antibody, zgc:77192 antibody, Cct4 antibody, Dmel\\CG5525 antibody, Tcp-1delta antibody, Tcp1-delta antibody, cct4 antibody, MGC89275 antibody, CCT4 antibody, DKFZp459D1424 antibody, LOC100225853 antibody, cctd antibody, CCT-DELTA antibody, Cctd antibody, SRB antibody, 2610204B21Rik antibody, A45 antibody, C78323 antibody, chaperonin containing TCP1, subunit 4 (delta) antibody, chaperonin containing TCP1 subunit 4 antibody, chaperonin containing TCP1 subunit 4 L homeolog antibody, CG5525 gene product from transcript CG5525-RA antibody, chaperonin containing Tcp1, subunit 4 (delta) antibody, T-complex protein 1 subunit delta antibody, cct4 antibody, CCT4 antibody, cct4.L antibody, CG5525 antibody, Cct4 antibody, cct-4 antibody

Background: The CCT (chaperonin containing TCP-1) complex functions as a molecular chaperone in the eukaryotic cytosol. CCT4 interacts with human cyclin E which has been implicated in positive control of the G1/S phase transition.
Alias Symbols: SRB, Cctd, CCT-DELTA
Protein Interaction Partner: HUWE1, TP53, FUS, CDC20, NEDD1, TUBG1, LGALS3BP, UBC, STAU1, MDM2, RFWD2, VRK2, PRPF40A, CCT7, USP9X, VARS, CCT3, CCT6A, FBXO6, UBD, FBXW4, CDK9, CDK5, CDK2, CD81, IGSF8, HDAC11, HDAC3, RPS6KA1, ILK, ICAM1, PAN2, VCAM1, PPP5C, PEX14, NOS2, US3, FAM86B2, C
Protein Size: 539

Molecular Weight: 58 kDa

Gene ID: 10575

NCBI Accession: NM_006430, NP_006421

UniProt: Q53QP9

Pathways: Embryonic Body Morphogenesis