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UBE2M antibody (N-Term)

ube2m Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2775686
  • Target See all UBE2M (ube2m) Antibodies
    UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
    Binding Specificity
    • 15
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 27
    • 16
    • 12
    • 10
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit
    Host
    • 39
    • 3
    Rabbit
    Clonality
    • 39
    • 3
    Polyclonal
    Conjugate
    • 11
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This UBE2M antibody is un-conjugated
    Application
    • 24
    • 13
    • 13
    • 11
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Sequence
    IKLFSLKQQK KEEESAGGTK GSSKKASAAQ LRIQKDINEL NLPKTCDISF
    Predicted Reactivity
    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
    Characteristics
    This is a rabbit polyclonal antibody against UBE2M. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human UBE2M
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 183 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

  • Target
    UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))
    Alternative Name
    UBE2M (ube2m Products)
    Synonyms
    DDBDRAFT_0204633 antibody, DDBDRAFT_0238042 antibody, DDB_0204633 antibody, DDB_0238042 antibody, UBC-RS2 antibody, UBC12 antibody, hUbc12 antibody, 2510040H03Rik antibody, Ubc-rs2 antibody, ubiquitin conjugating enzyme E2M antibody, ubiquitin-conjugating enzyme E2M antibody, ubiquitin conjugating enzyme E2 M antibody, NEDD8-conjugating enzyme antibody, ubiquitin conjugating enzyme E2M S homeolog antibody, ube2m antibody, LOC692934 antibody, UBE2M antibody, Ube2m antibody, ube2m.S antibody
    Background
    The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. It is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin.
    Alias Symbols: UBC-RS2, UBC12, hUbc12
    Protein Interaction Partner: DCUN1D1, DCUN1D4, RBX1, UBC, MARCH7, UBE2M, CUL1, DCUN1D3, SMURF1, CAND1, DCUN1D2, NEDD8, DCUN1D5, LMNA, VCAM1, ITGA4, FN1, VCP, UBE2D2, TYMS, TST, VAMP2, PARK2, PINK1, UBE2T, SEC22B, SLC9A3R2, APP, CUL3, CUL4A, TP53, UBD, COPS6, UBA3, NAE1, CUL2, Nedd4,
    Protein Size: 183
    Molecular Weight
    21 kDa
    Gene ID
    9040
    NCBI Accession
    NM_003969, NP_003960
    UniProt
    P61081
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