UBE2E1 antibody (Middle Region)

Catalog No. ABIN2775676

Product Details anti-UBE2E1 Antibody

Target: UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rabbit, Dog, Guinea Pig, Rat, Horse, Cow, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2E1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PKGDNIYEWR STILGPPGSV YEGGVFFLDI TFTPEYPFKP PKVTFRTRIY

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against UBE2E1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2E1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 193 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBE2E1

Target: UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))

Alternative Name: UBE2E1 (UBE2E1 Products)

Synonyms: ubch6 antibody, UBCH6 antibody, UbcM3 antibody, Ubce5 antibody, ubcM2 antibody, ubiquitin conjugating enzyme E2 E1 antibody, ubiquitin-conjugating enzyme E2 E1 antibody, ubiquitin-conjugating enzyme E2E 1 antibody, ubiquitin conjugating enzyme E2 E1 L homeolog antibody, ube2e1 antibody, UBE2E1 antibody, LOC100354960 antibody, LOC100362535 antibody, Ube2e1 antibody, ube2e1.L antibody

Background: UBE2E1 catalyzes the covalent attachment of ubiquitin to other proteins. UBE2E1 mediates the selective degradation of short-lived and abnormal proteins.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: UBCH6
Protein Interaction Partner: TRIM33, RNF11, TRIM32, UBC, TRAF6, MID1, TRIM23, XIAP, NHLRC1, ZNRF1, JADE1, RNF26, SLX8, SLX5, MARCH7, UBE2E1, MARCH5, RNF167, UBA1, RNF130, USP7, NEDD4, Rag1, ZNF827, PARK2, BRCA1, RNF122, RNF128, ITCH, TRIM39, RNF20, FBXO2, WWP2, STUB1, TOPORS, TRIM28,
Protein Size: 193

Molecular Weight: 21 kDa

Gene ID: 7324

NCBI Accession: NM_003341, NP_003332

UniProt: P51965

Pathways: Ubiquitin Proteasome Pathway