UBE2D2 antibody (Middle Region)

Catalog No. ABIN2775673

Product Details anti-UBE2D2 Antibody

Target: UBE2D2 (Ubiquitin-Conjugating Enzyme E2D 2 (UBE2D2))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Zebrafish (Danio rerio), Horse, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBE2D2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: SQWSPALTIS KVLLSICSLL CDPNPDDPLV PEIARIYKTD REKYNRIARE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against UBE2D2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE2D2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 547 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-UBE2D2 antibody (ABIN2775673)

Desforges, Lacey, Glazier, Greenwood, Mynett, Speake, Sibley: "SNAT4 isoform of system A amino acid transporter is expressed in human placenta." in: American journal of physiology. Cell physiology, Vol. 290, Issue 1, pp. C305-12, (2005) (PubMed).

Target Details for UBE2D2

Target: UBE2D2 (Ubiquitin-Conjugating Enzyme E2D 2 (UBE2D2))

Alternative Name: UBE2D2 (UBE2D2 Products)

Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. UBE2D2 is a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase.SLC38A4 is found predominantly in liver and transports both cationic and neutral amino acids. The transport of cationic amino acids by SLC38A4 is Na(+) and pH independent, while the transport of neutral amino acids is Na(+) and pH dependent (Hatanaka et al., 2001).[supplied by OMIM].
Alias Symbols: E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH5B
Protein Interaction Partner: RNF26, CHFR, RBX1, DZIP3, UBA1, UBC, TRAF6, BIRC7, anapc11, ZNRF1, RNF146, MID1, MDM2, BIRC3, FZR1, ITCH, HRD1, CUL3, XIAP, MARCH7, RNF25, UBE2D2, RNF130, UBA6, CBLC, PARK2, TRIM21, KCMF1, RNF135, MUL1, MDM4, TRIM23, STUB1, CBL, UBTD1, RNF38, UBI4, RFFL,
Protein Size: 547

Molecular Weight: 60 kDa

Gene ID: 7322

NCBI Accession: NM_018018, NP_003330

UniProt: P62837

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades