RFFL antibody (Middle Region)

Catalog No. ABIN2775666

Product Details anti-RFFL Antibody

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Horse, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFFL antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KDQKGLQHLV SGAEDQNGGA VPSGLEENLC KICMDSPIDC VLLECGHMVT

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against RFFL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RFFL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 363 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-RFFL antibody (ABIN2775666)

Hanuszkiewicz, Pittock, Humphries, Moll, Rosales, Molinaro, Moynagh, Lajoie, Valvano: "Identification of the flagellin glycosylation system in Burkholderia cenocepacia and the contribution of glycosylated flagellin to evasion of human innate immune responses." in: The Journal of biological chemistry, Vol. 289, Issue 27, pp. 19231-44, (2014) (PubMed).

Target Details for RFFL

Target: RFFL (Ring Finger and FYVE-Like Domain Containing 1 (RFFL))

Alternative Name: RFFL (RFFL Products)

Background: RFFL has E3 ubiquitin protein ligase activity.RFFL regulates the levels of CASP8 and CASP10 by targeting them for proteasomal degradation. Has anti-apoptotic activity.RFFL may bind phosphatidylinositol phosphates.
Alias Symbols: RNF189, RNF34L, CARP2, FRING, CARP-2, RIFIFYLIN
Protein Interaction Partner: RIPK1, UBE2D1, UBC, UBE2D2, RFFL, ATXN3L, OTUB2, USP28, OTUB1, UCHL5, ESPL1, USP5, STAM, USP7, UBE2D3, ATXN3, TP53, MDM2, UBE2G2,
Protein Size: 363

Molecular Weight: 40 kDa

Gene ID: 117584

NCBI Accession: NM_001017368, NP_001017368

UniProt: Q8WZ73