ube3a antibody (Middle Region)

Catalog No. ABIN2775648

Product Details anti-ube3a Antibody

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ube3a antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AKNGPDTERL PTSHTCFNVL LLPEYSSKEK LKERLLKAIT YAKGFGML

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against UBE3A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human UBE3A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 875 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ube3a

Target: ube3a (Ubiquitin Protein Ligase E3A (ube3a))

Alternative Name: UBE3A (ube3a Products)

Background: UBE3A is an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53.Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined.
Alias Symbols: ANCR, AS, E6-AP, EPVE6AP, FLJ26981, HPVE6A
Protein Interaction Partner: KLHL38, UBE2D1, UBB, TP53, TAT, AFG3L1P, ATG9A, ASAP3, AHSP, UBE3A, UBC, MEOX2, MAGEA8, JADE1, YOD1, UBE2L3, UBE2D3, PSMD14, SHFM1, ARNTL, LPP, HSF1, DR1, BTF3L4, LRSAM1, P3H1, NPLOC4, IPO11, DBNL, LSM1, ARPC1B, METTL1, SACS, ARC, RAD23A, PTPN3, CEBPA, SC
Protein Size: 875

Molecular Weight: 101 kDa

Gene ID: 7337

NCBI Accession: NM_000462, NP_000453

UniProt: Q05086

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway