AMOTL1 antibody (N-Term)

Catalog No. ABIN2775636

Product Details anti-AMOTL1 Antibody

Target: AMOTL1 (Angiomotin Like 1 (AMOTL1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Cow, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AMOTL1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LTQEDPQMVY QSARQEPQGQ EHQVDNTVME KQVRSTQPQQ NNEELPTYEE

Predicted Reactivity: Cow: 93%, Dog: 79%, Guinea Pig: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%

Characteristics: This is a rabbit polyclonal antibody against AMOTL1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AMOTL1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 956 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AMOTL1 antibody (ABIN2775636)

Skouloudaki, Walz: "YAP1 recruits c-Abl to protect angiomotin-like 1 from Nedd4-mediated degradation." in: PLoS ONE, Vol. 7, Issue 4, pp. e35735, (2012) (PubMed).

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for AMOTL1

Target: AMOTL1 (Angiomotin Like 1 (AMOTL1))

Alternative Name: AMOTL1 (AMOTL1 Products)

Synonyms: JEAP antibody, 2310010G08Rik antibody, 2310067L22Rik antibody, 4932416D09Rik antibody, mFLJ00155 antibody, angiomotin like 1 antibody, angiomotin-like 1 antibody, AMOTL1 antibody, Amotl1 antibody

Background: AMOTL1 is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation.The protein encoded by this gene is a peripheral membrane protein that is a component of tight junctions or TJs. TJs form an apical junctional structure and act to control paracellular permeability and maintain cell polarity. This protein is related to angiomotin, an angiostatin binding protein that regulates endothelial cell migration and capillary formation.
Alias Symbols: JEAP
Protein Interaction Partner: WWOX, SUZ12, NEDD4, HECW2, AMOT, LATS2, YAP1, LATS1, Wwtr1, UBC, Magi1, NEDD4L,
Protein Size: 956

Molecular Weight: 106 kDa

Gene ID: 154810

NCBI Accession: NM_130847, NP_570899

UniProt: Q8IY63