PARD3 antibody (Middle Region)

Catalog No. ABIN2775627

Product Details anti-PARD3 Antibody

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Binding Specificity: Middle Region

Reactivity: Human, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: EQQMKKQPPS EGPSNYDSYK KVQDPSYAPP KGPFRQDVPP SPSQVARLNR

Predicted Reactivity: Guinea Pig: 79%, Human: 100%

Characteristics: This is a rabbit polyclonal antibody against PARD3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PARD3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1356 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for PARD3

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Alternative Name: PARD3 (PARD3 Products)

Background: PARD proteins, which were first identified in C. elegans, are essential for asymmetric cell division and polarized growth, whereas CDC42 (MIM 116952) mediates the establishment of cell polarity. The CDC42 GTPase, which is controlled by nucleotide exchange
Alias Symbols: ASIP, Baz, Bazooka, FLJ21015, PAR3, PAR3alpha, PARD3A, SE2-5L16, SE2-5LT1, SE2-5T2
Protein Interaction Partner: UBC, AMOTL2, LATS2, YAP1, LATS1, YWHAB, INADL, PRKCI, GAB1, MARK2, PARD6A, YWHAE, AMOT, ARHGAP17, VHL, TJP1, Smurf1, Smurf2, ANP32E, PSME3, RPS18, RPS3, PSME2, PFKP, KPNA4, HSPD1, HSPA8, HSPA6, SIAH1, PARD6B, PARD6G, CCDC88A, RASSF8, PNMA2, DCAF7, PNMA1,
Protein Size: 1356

Molecular Weight: 151 kDa

Gene ID: 56288

NCBI Accession: NM_019619, NP_062565

UniProt: Q8TEW0

Pathways: Cell-Cell Junction Organization