COX IV antibody (N-Term)

Catalog No. ABIN2775574

Product Details anti-COX IV Antibody

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COX IV antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: AISTSVCVRA HESVVKSEDF SLPAYMDRRD HPLPEVAHVK HLSASQKALK

Predicted Reactivity: Cow: 86%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 85%, Rabbit: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against COX4I1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human COX4I1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 169 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-COX IV antibody (ABIN2775574)

Hwang, Dornbos, Steidemann, Dunivin, Rizzo, LaPres: "Mitochondrial-targeted aryl hydrocarbon receptor and the impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin on cellular respiration and the mitochondrial proteome." in: Toxicology and applied pharmacology, (2016) (PubMed).

Geerling, Boon, van der Zon, van den Berg, van den Hoek, Lombès, Princen, Havekes, Rensen, Guigas: "Metformin lowers plasma triglycerides by promoting VLDL-triglyceride clearance by brown adipose tissue in mice." in: Diabetes, Vol. 63, Issue 3, pp. 880-91, (2014) (PubMed).

Snyder, Wang, Patton, Arosio, Levi, Earley, Allen, Connor: "Mitochondrial ferritin in the substantia nigra in restless legs syndrome." in: Journal of neuropathology and experimental neurology, Vol. 68, Issue 11, pp. 1193-9, (2009) (PubMed).

Target Details for COX IV

Target: COX IV (COX4I1) (Cytochrome C Oxidase Subunit IV Isoform 1 (COX4I1))

Alternative Name: COX4I1 (COX4I1 Products)

Synonyms: COX4 antibody, COX4-1 antibody, COXIV antibody, mg:bb02d03 antibody, zgc:110058 antibody, GB20012 antibody, AL024441 antibody, Cox4 antibody, Cox4a antibody, cox4 antibody, cox4i1 antibody, coxiv antibody, BcDNA:AT07685 antibody, CG10396 antibody, COX IV antibody, CX41 antibody, CX42 antibody, Dmel\CG10396 antibody, IV antibody, cytochrome c oxidase subunit 4I1 antibody, cytochrome c oxidase subunit IV isoform 1 antibody, cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody, cytochrome c oxidase polypeptide IV antibody, cytochrome c oxidase subunit IV antibody, cytochrome c oxidase subunit 4i1 antibody, cytochrome c oxidase subunit IV isoform 1 S homeolog antibody, Cytochrome c oxidase subunit 4-like antibody, COX4I1 antibody, cox4i1 antibody, LOC412396 antibody, Cox4I1 antibody, LOC780848 antibody, BMEII0322 antibody, RSal33209_1582 antibody, SJAG_00976 antibody, HMPREF0733_11926 antibody, Cox4i1 antibody, cox4i1.S antibody, COX4L antibody

Background: Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. COX4I1 is the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme.Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.
Alias Symbols: COX4, COXIV, MGC72016, COX4-1
Protein Interaction Partner: SDCBP, COX1, env, APP, UBC, ICT1, TMBIM4, NELFCD,
Protein Size: 169

Molecular Weight: 19 kDa

Gene ID: 1327

NCBI Accession: NM_001861, NP_001852

UniProt: P13073

Pathways: Proton Transport