PLOD2 antibody (Middle Region)

Catalog No. ABIN2775556

Product Details anti-PLOD2 Antibody

Target: PLOD2 (Procollagen-Lysine 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Cow, Dog, Horse, Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PLOD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: IKGKTLRSEM NERNYFVRDK LDPDMALCRN AREMTLQREK DSPTPETFQM

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PLOD2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human PLOD2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 758 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PLOD2 antibody (ABIN2775556)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for PLOD2

Target: PLOD2 (Procollagen-Lysine 2-Oxoglutarate 5-Dioxygenase 2 (PLOD2))

Alternative Name: PLOD2 (PLOD2 Products)

Synonyms: D530025C14Rik antibody, LH2 antibody, Plod-2 antibody, TLH antibody, procollagen lysine, 2-oxoglutarate 5-dioxygenase 2 antibody, procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 antibody, Plod2 antibody, PLOD2 antibody

Background: PLOD2 forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms.
Alias Symbols: LH2, TLH
Protein Interaction Partner: ITGB1BP2, SUPT5H, CSDE1, SF1, UGP2, TPP2, VASN, RNF123, NUSAP1, SAMHD1, DNPEP, GANAB, SWAP70, XPO7, PACSIN2, FAF1, HSPH1, SEC23A, PRMT3, FARSB, USP15, RANBP3, SET, RPL24, PTPN12, NCL, NARS, JUP, GART, XRCC6, AP2B1, gag-pol, UBL4A, OS9, FBXO6, UBC, VHL, Fa
Protein Size: 758

Molecular Weight: 84 kDa

Gene ID: 5352

NCBI Accession: NM_182943, NP_891988

Pathways: SARS-CoV-2 Protein Interactome