PPP4R2 antibody (C-Term)

Catalog No. ABIN2775552

Product Details anti-PPP4R2 Antibody

Target: PPP4R2 (Protein Phosphatase 4, Regulatory Subunit 2 (PPP4R2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPP4R2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DSRCTRQHCT EEDEEEDEEE EEESFMTSRE MIPERKNQEK ESDDALTVNE

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against PPP4R2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PPP4R2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 417 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PPP4R2 antibody (ABIN2775552)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for PPP4R2

Target: PPP4R2 (Protein Phosphatase 4, Regulatory Subunit 2 (PPP4R2))

Alternative Name: PPP4R2 (PPP4R2 Products)

Background: PPP4R2 is the regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). It may regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA DSB repair.
Alias Symbols: MGC131930, PP4R2
Protein Interaction Partner: UBC, PAAF1, SMEK1, SF3A1, CCDC6, VASP, CCT3, PSMA1, INPPL1, AMPD2, LRRC47, DSTN, PLIN3, NUBP2, TPM1, TRA2B, IGBP1, CLNS1A, PPP4C, SRRM2, TIPRL, SMEK2, GEMIN4, DDX20,
Protein Size: 417

Molecular Weight: 47 kDa

Gene ID: 151987

NCBI Accession: NM_174907, NP_777567

UniProt: Q9NY27