ANO1 antibody (Middle Region)

Catalog No. ABIN2775453

Product Details anti-ANO1 Antibody

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANO1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: HGFVNHTLSS FNVSDFQNGT APNDPLDLGY EVQICRYKDY REPPWSENKY

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 79%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against TMEM16A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TMEM16A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 960 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ANO1 antibody (ABIN2775453)

Liu, Zhang, Huang, Qi, Xu, Gao, Du, Gamper, Zhang: "Characterization of the effects of Cl⁻ channel modulators on TMEM16A and bestrophin-1 Ca²⁺ activated Cl⁻ channels." in: Pflügers Archiv : European journal of physiology, Vol. 467, Issue 7, pp. 1417-1430, (2015) (PubMed).

Banga, Flaig, Lewis, Winfree, Blazer-Yost: "Epinephrine stimulation of anion secretion in the Calu-3 serous cell model." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 306, Issue 10, pp. L937-46, (2014) (PubMed).

Target Details for ANO1

Target: ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))

Alternative Name: TMEM16A (ANO1 Products)

Synonyms: DOG1 antibody, ORAOV2 antibody, TAOS2 antibody, TMEM16A antibody, Tmem16a antibody, anoctamin 1 antibody, anoctamin 1, calcium activated chloride channel antibody, Ano1 antibody, ANO1 antibody

Background: TMEM16A belongs to the anoctamin family. TMEM16A acts as a calcium-activated chloride channel. It is required for normal tracheal development.
Alias Symbols: FLJ10261, ORAOV2, TAOS2, CACC, DOG1, TMEM16A, ANO1
Protein Interaction Partner: UBC,
Protein Size: 960

Molecular Weight: 111 kDa

Gene ID: 55107

NCBI Accession: NM_018043, NP_060513

UniProt: Q5XXA6