NAMPT antibody (C-Term)

Catalog No. ABIN2775322

Product Details anti-NAMPT Antibody

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Dog, Guinea Pig, Cow, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NAMPT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA QLNIELEAAH

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 93%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against PBEF1. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human PBEF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 491 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NAMPT antibody (ABIN2775322)

Xia, Weisenburger, Koch, Burkart, Reifenberg, Förstermann, Li: "Restoration of perivascular adipose tissue function in diet-induced obese mice without changing bodyweight." in: British journal of pharmacology, Vol. 174, Issue 20, pp. 3443-3453, (2017) (PubMed).

Target Details for NAMPT

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: PBEF1 (NAMPT Products)

Background: PBEF1 catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It also activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Alias Symbols: 1110035O14Rik, DKFZP666B131, MGC117256, NAMPT, PBEF, VF, PBEF1, VISFATIN
Protein Interaction Partner: UBC, NEDD8, NDRG1, CTSA, PEPD, MVD, GSR, ASS1, ANXA6, CD81, AKT2, ADORA2A, IFITM3, ND1, GGT1, FTL, CUL5, DDA1, GBAS, FANCA,
Protein Size: 491

Molecular Weight: 54 kDa

Gene ID: 10135

NCBI Accession: NM_005746, NP_005737

UniProt: P43490