SLC1A5 antibody (Middle Region)

Catalog No. ABIN2775316

Product Details anti-SLC1A5 Antibody

Target: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow, Sheep, Horse, Guinea Pig, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC1A5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: FGVALRKLGP EGELLIRFFN SFNEATMVLV SWIMWYAPVG IMFLVAGKIV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against SLC1A5. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SLC1A5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 541 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SLC1A5 antibody (ABIN2775316)

Qiu, Qin, Luo, Zhang, Sun, Jiao, Li, Yin: "Protein Restriction with Amino Acid-Balanced Diets Shrinks Circulating Pool Size of Amino Acid by Decreasing Expression of Specific Transporters in the Small Intestine." in: PLoS ONE, Vol. 11, Issue 9, pp. e0162475, (2016) (PubMed).

Zhu, Lin, Dai, Zhou, Li, Yuan, Wu, Wu, Wang: "L-Glutamine deprivation induces autophagy and alters the mTOR and MAPK signaling pathways in porcine intestinal epithelial cells." in: Amino acids, Vol. 47, Issue 10, pp. 2185-97, (2015) (PubMed).

Bungard, McGivan: "Identification of the promoter elements involved in the stimulation of ASCT2 expression by glutamine availability in HepG2 cells and the probable involvement of FXR/RXR dimers." in: Archives of biochemistry and biophysics, Vol. 443, Issue 1-2, pp. 53-9, (2005) (PubMed).

Target Details for SLC1A5

Target: SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))

Alternative Name: SLC1A5 (SLC1A5 Products)

Synonyms: aaat antibody, asct2 antibody, atbo antibody, m7v1 antibody, m7vs1 antibody, r16 antibody, rdrc antibody, AAAT antibody, ASCT2 antibody, ATBO antibody, M7V1 antibody, M7VS1 antibody, R16 antibody, RDRC antibody, Slc1a7 antibody, Asct2 antibody, H4-ASCT2 antibody, solute carrier family 1 (neutral amino acid transporter), member 5 antibody, solute carrier family 1 member 5 S homeolog antibody, solute carrier family 1 member 5 antibody, slc1a5 antibody, slc1a5.S antibody, SLC1A5 antibody, Slc1a5 antibody

Background: SLC1A5 has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated amino acids, anionic amino acids, and cationic amino acids. It acts as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses.
Alias Symbols: AAAT, ASCT2, ATBO, FLJ31068, M7V1, M7VS1, R16, RDRC
Protein Interaction Partner: UBC, FUS, LGR4, ADRB2, ALB, PAXIP1, ECT2, MCU, MRPS35, CYB5R3, CAND1, CUL3, USP50, USP19, TBC1D17, ERVW-1, NEK6, MPDU1, LGALS9,
Protein Size: 541

Molecular Weight: 56 kDa

Gene ID: 6510

NCBI Accession: NM_005628, NP_005619

UniProt: Q71UA6

Pathways: Dicarboxylic Acid Transport, Warburg Effect