DNA Helicase B antibody (N-Term)

Catalog No. ABIN2775262

Product Details anti-DNA Helicase B Antibody

Target: DNA Helicase B (HELB)

Binding Specificity: N-Term

Reactivity: Human, Pig, Cow, Guinea Pig, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNA Helicase B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FGRFPITGAW WRVKVQVKPV VGSRSYQYQV QGFPSYFLQS DMSPPNQKHI

Predicted Reactivity: Cow: 86%, Guinea Pig: 79%, Human: 100%, Pig: 86%, Rat: 86%

Characteristics: This is a rabbit polyclonal antibody against HELB. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human HELB

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1087 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DNA Helicase B antibody (ABIN2775262)

Taneja, Gu, Peng, Carrick, Uchiumi, Ott, Gustafson, Podust, Fanning: "A dominant-negative mutant of human DNA helicase B blocks the onset of chromosomal DNA replication." in: The Journal of biological chemistry, Vol. 277, Issue 43, pp. 40853-61, (2002) (PubMed).

Target Details for DNA Helicase B

Target: DNA Helicase B (HELB)

Alternative Name: HELB (HELB Products)

Synonyms: RGD1560103 antibody, hDHB antibody, AI447783 antibody, D10Ertd664e antibody, DNA helicase B antibody, helicase (DNA) B antibody, Helb antibody, HELB antibody

Background: The protein encoded by this gene is a DNA helicase. A dominant-negative mutant of this protein blocks chromosomal DNA replication and suggests that its function is required for S phase entry.
Alias Symbols: hDHB
Protein Interaction Partner: UBC, PMS1, POLA2, POLA1, RPA1,
Protein Size: 1087

Molecular Weight: 120 kDa

Gene ID: 92797

NCBI Accession: NM_033647, NP_387467

UniProt: Q8NG08