DDX27 antibody (N-Term)

Catalog No. ABIN2775237

Product Details anti-DDX27 Antibody

Target: DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX27 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DLGLIGTIGE DDEVPVEPES DSGDEEEEGP IVLGRRQKAL GKNRSADFNP

Predicted Reactivity: Dog: 79%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against DDX27. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DDX27

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 796 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-DDX27 antibody (ABIN2775237)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for DDX27

Target: DDX27 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 27 (DDX27))

Alternative Name: DDX27 (DDX27 Products)

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX27 is a DEAD box protein, the function of which has not been determined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, the function of which has not been determined.
Alias Symbols: DKFZp667N057, FLJ12917, FLJ20596, FLJ22238, HSPC259, MGC1018, MGC163147, PP3241, RHLP, Rrp3p, dJ686N3.1, DRS1
Protein Interaction Partner: SUMO3, STAU1, UBC, TARBP2, EED, RNF2, HDAC11, ESR1, API5, SIRT7, YWHAG, PPP1R16A, LYAR, ARRB2, ARRB1,
Protein Size: 796

Molecular Weight: 90 kDa

Gene ID: 55661

NCBI Accession: NM_017895, NP_060365

UniProt: Q96GQ7